Cell-type-specific nuclei purification from whole animals for genome-wide expression and chromatin profiling

摘要: An understanding of developmental processes requires knowledge transcriptional and epigenetic landscapes at the level tissues ultimately individual cells. However, obtaining tissue- or cell-type-specific expression chromatin profiles for animals has been challenging. Here we describe a method purifying nuclei from specific cell types animal models that allows simultaneous determination both profiles. The is based on in vivo biotin-labeling nuclear envelope subsequent affinity purification nuclei. We use to isolate muscle adult Caenorhabditis elegans mesoderm Drosophila melanogaster embryos. As case study, determined nucleosome occupancy affinity-purified C. muscle. identified hundreds genes are specifically expressed found these depleted nucleosomes promoters gene bodies relative other tissues. This should be universally applicable all model systems allow transgenesis will make it possible determine different types.