Inherent differences in nasal and tracheal ciliary function in response to Pseudomonas aeruginosa challenge.

摘要: BACKGROUND: Sinonasal mucosal biofilms are recognized as contributors to the pathogenesis of chronic rhinosinusitis (CRS). Attachment bacteria sinonasal surface is an initial step in biofilm formation. A critical defense against this occurrence mucociliary clearance (MCC). To ascertain whether ciliary component MCC uniform throughout airway we compared beat frequency (CBF) murine nasal septum and trachea at baseline after challenge with Pseudomonas aeruginosa, a common pathogen CRS. METHODS: Murine septal tracheal air-liquid interface cultures were evaluated for basal stimulated CBF exposure control or conditioned media from Pseudomonas. Additionally, attachment was assessed pretreatment media. RESULTS: Basal significantly slower primary cultures. Tracheal show resistance secreted ciliotoxins not evident Furthermore, viable Pseudomonas, more attach CONCLUSION: Using inherent differences cilia function increased susceptibility upper by Understanding between subglottic should lead novel approaches management infection.