Enhancements and modifications of primer design program Primer3
作者： T. Koressaar , M. Remm
DOI: 10.1093/BIOINFORMATICS/BTM091
关键词: Binary code 、 Primer (molecular biology) 、 Simulation 、 Computer science 、 Programming language
摘要: … in PCR design. This parameter is typically derived from the melting temperature of the PCR primers, so for successful PCR work it is important to determine the melting temperature of …
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