Simultaneously detection of genomic and expression alterations in prostate cancer using cDNA microarray

作者: Mei Jiang , Ming Li , Xuping Fu , Yan Huang , Hui Qian

DOI: 10.1002/PROS.20756

关键词: Protein ubiquitinationBiologyGene expression profilingProstate cancerGene expressionGeneComparative genomic hybridizationDNA microarrayGeneticsMicroarrayUrologyOncology

摘要: BACKGROUND Prostate cancer is a common disease among men but the knowledge of prostate carcinogenesis still limited. METHODS cDNA microarray-based comparative genomic hybridization (CGH) and expression profiling were performed to screen changes in respectively. The two data integrated study influence aberrations on gene seek for genes with their affected by aberrations. Real-time PCR was evaluate array data. RESULTS Array-based CGH detected gains at 2q, 3p/q, 5q, 6q, 8q, 9p, 10p/q, 11q, 12p, 14q, 19p/q losses 1p, 2p, 4q, 6p/q, 7p, 11p/q, 12q, 17p/q, 19p/q, Xp/q more than 20% tumors narrowed these For example, gain 8q mapped five minimal regions. Novel also identified, such as loss Xq21.33-q22.2. Expression discovered significant biological processes involved carcinogenesis, exogenous antigen presentation via MHC class II protein ubiquitination. Integration analysis revealed weak positive correlation between copy number level. Fifty-three showed directly possibly, including one member Ras superfamily major histocompatibility complex (MHC). These are multiple processes. CONCLUSIONS Integration provided information separate analysis. Although direct seems weak, can be extended indirect regulation through few genes. Because persistent, may play key roles worth further Prostate 68: 1496–1509, 2008. © 2008 Wiley-Liss, Inc.

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