Derivation and comparison of C57BL/6 embryonic stem cells to a widely used 129 embryonic stem cell line

摘要: Typically, embryonic stem (ES) cells derived from 129 mouse substrains are used to generate genetically altered models. Resulting chimeric mice were then usually converted a C57BL/6 background, which takes at least year, even in the case of speed congenics. In recent years, have been various strains. However, ES still widely partially due poor germline transmission other Availability highly germline-competent would enormously facilitate generation pure genetic background by eliminating backcrossing time, and thus significantly reducing associated costs efforts. Here, we describe establishment cell line (LK1) compare its efficacy 129SvJ (GSI-1) generating chimeras. contrast earlier studies, our data shows that lines can be using simple approach, support broader use for engineered