Detection of preferential NRAS mutations in human male germ cell tumors by the polymerase chain reaction.
作者： Sabyasachi Ganguly , Vundavalli V. V. S. Murty , Felipe Samaniego , Victor E. Reuter , George J. Bosl
关键词: Gene mutation 、 HRAS 、 Embryonal carcinoma 、 Germ cell tumors 、 Teratoma 、 KRAS 、 Biology 、 Neuroblastoma RAS viral oncogene homolog 、 Molecular biology 、 Mutation
摘要: We have studied 31 male germ cell tumors (GCTs) for probable mutations in codons 12, 13, and 61 of HRAS, KRAS, NRAS oncogenes using the polymerase chain reaction. Twenty thirty-one exhibited gene mutations, 14 codon 61, six whereas no were detected HRAS KRAS genes. The equally prevalent seminomatous nonseminomatous GCTs. Thus 13 22 seminomas, seven embryonal carcinomas, one two mixed mutations. Two non-seminomatous (an carcinoma a yolk sac/teratoma) had both 12 61. high frequency observed present study suggests that products may play an important role growth regulatory functions premalignant malignant cells.
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