A dot-blot screening procedure for mutated ras oncogenes using synthetic oligodeoxynucleotides.

作者: Matty Verlaan-de Vries , Marianne E. Bogaard , Hans van den Elst , Jaques H. van Boom , Alex J. van der Eb

DOI: 10.1016/0378-1119(86)90335-5

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摘要: Abstract To analyze human tumors for the presence of mutated ras oncogenes, a procedure was developed based on selective hybridization mutation-specific oligodeoxynucleotide probes to genomic DNA [Bos et al., Nucl. Acids Res. 12 (1984) 9155–9163]. We have improved this both in sensitivity and speed by including an vitro amplification step -specific sequences. This has first been described Saiki al. [ Science 230 (1985) 1350–1353] results more than 10 4 -fold increase sequence which might contain mutation. Furthermore, we selectivity our hybridizations. As result, oncogenes can now be detected with dot-blot screening requiring less 1 μg tumor DNA.

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