Transmission of Mycobacterium tuberculosis undetected by tuberculin skin testing.

作者: Suzanne T. Anderson , Amanda J. Williams , Jillian R. Brown , Sandra M. Newton , Marcela Simsova

DOI: 10.1164/RCCM.200509-1526OC

关键词: AntigenTuberculosisImmunologyTransmission (medicine)Mycobacterium tuberculosisVirologyIntensive careBordetella pertussisMedicineCytokine secretionTuberculin

摘要: Rationale: The development of tuberculin skin test (TST) positivity following infection by Mycobacterium tuberculosis is not invariable and may depend on bacillary as well host factors.Objectives: First, to compare the diagnostic performance TST a form in vitro IFN-γ release assay (IFNGRA) circumstances contact investigation prompted an unusually severe index case infectious pulmonary tuberculosis. Second, investigate ability strain M. responsible induce cytokine secretion from monocytes vitro.Methods: A routine TST-based tuberculosis-contact screening procedure supplemented use “in house” IFNGRA that assays T-cell response tuberculosis–specific antigens ESAT-6, CFP-10 (presented fusion protein within inactivated adenylate cyclase Bordetella pertussis), purified derivative Isolation genetic typing responsible, o...

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