HBT-based turn-on fluorescent probe for discrimination of homocysteine from glutathione/cysteine and its bioimaging applications
作者： Xiuhua Zhang , Shengfu Wang , Fengzao Chen , Zhen Chen , Yuanchao Sun
DOI: 10.1039/C6RA28712C
关键词: Selectivity 、 Glutathione 、 Cysteine 、 Fluorescence-lifetime imaging microscopy 、 Fluorescence 、 Detection limit 、 Biophysics 、 HeLa 、 Analytical chemistry 、 Chemistry 、 Turn (biochemistry)
摘要: Based on ESIPT and heavy atom effect strategy, we firstly reported a new type of turn-on fluorescent probe for Hcy. This displayed fast response time (15 min), excellent selectivity over other interfering species high sensitivity with low detection limit 1.6 × 10−7 M in PBS buffer containing 1% CH3CN (v/v). Moreover, the was also successfully applied fluorescence imaging Hcy HeLa cells.
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