Membranes and their constituents as promoters of calcium oxalate crystal formation in human urine.

摘要: We have proposed that membranes of cellular degradation products are a suitable substrate for the nucleation calcium oxalate (CaOx) crystals in human urine. Human urine is generally metastable with respect to CaOx. To demonstrate present promote CaOx we removed these substrates by filtration or centrifugation and induced crystallization adding sodium oxalate, before after centrifugation. In separate experiment, membrane vesicles isolated from rat renal tubular brush border were added into filtered centrifuged crystal induction. Crystals counted using particle counter. Urine, pellet, retentate analyzed presence membranes, lipids, proteins. Lipids further separated different classes, identified, quantified. Both proteins, vesicles, causing reduction lipid protein contents More formed whole than The number significantly increased when was supplemented various urinary components such as phospholipids, which during filtration. also determined limit Filtration associated reduced addition vesicles. These results support our hypothesis normally contains promoters formation their constituents most likely