Intratubular crystallization of calcium oxalate in the presence of membrane vesicles: An in vitro study

摘要: Intratubular crystallization of calcium oxalate in the presence membrane vesicles: An vitro study. Background Since urine spends only a few minutes renal tubules and has low supersaturation with respect to (CaOx), nucleation CaOx crystals kidneys is most probably heterogeneous. We have proposed that membranes cellular degradation products are main substrate for crystal nucleation. The purpose our study was determine site membrane-mediated within required lag time, factors whether results crystalluria or nephrolithiasis. Methods Nucleation allowed occur five different artificial solutions ionic concentrations simulating proximal (PTs), descending (DLH) ascending (ALH) limbs loop Henle, distal (DTs), collecting ducts (CDs). A constant composition system used. Experiments were run two hours without tubular brush border (BBM) vesicles. Results addition BBM significantly reduced time increased rate crystallization. average decreased from 84.6 ± 43.4 24.5 19 PTs, 143.6 29 70.2 53.4 DLH, 17.6 8.6 0.625 0.65 DTs 9.54 3.03 CDs. There no ALH solution hours. dihydrate (COD) common solutions. Calcium phosphate (CaP) also nucleated DLH CD Conclusions In absence vesicles, there any tubules. As result, homogeneous anywhere nephron appears unlikely. However, BBM-supported possible as well high CDs would promote rapid growth aggregation, resulting retention development