The calcium signal and phosphatidylinositol breakdown in 2H3 cells.

作者: M A Beaven , J P Moore , G A Smith , T R Hesketh , J C Metcalfe

DOI: 10.1016/S0021-9258(17)39848-4

关键词: BiochemistryPiHistamineStimulationCalciumPhosphatidylinositolIonophoreChemistryPhosphorylationPhosphodiesteraseBiophysics

摘要: Phosphatidylinositol (PI) and its phosphorylated derivatives are rapidly broken down in 2H3 cells stimulated with antigen, a time course which coincides the generation of Ca signal. Stimulated PI breakdown is absolutely dependent on Ca2+ medium concentration dependence similar to that signal histamine release described preceding paper. However, does not depend rise free cytoplasmic over range 100 nM 1 microM. Thus, stimulation by ionophore A23187 causes only small increase antigen can be selectively blocked appropriate concentrations Zn2+ (100 microM) or La3+ (10-100 have negligible effects breakdown. Both appear common external site (or sites) Km approximately equal 0.4 mM, data consistent either independent activation phosphodiesterase after antigenic stimulation, as component mechanism generated.

参考文章(23)
M A Beaven, J Rogers, J P Moore, T R Hesketh, G A Smith, J C Metcalfe, The mechanism of the calcium signal and correlation with histamine release in 2H3 cells. Journal of Biological Chemistry. ,vol. 259, pp. 7129- 7136 ,(1984) , 10.1016/S0021-9258(17)39847-2
M J Berridge, R M C Dawson, C P Downes, J P Heslop, R F Irvine, Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides. Biochemical Journal. ,vol. 212, pp. 473- 482 ,(1983) , 10.1042/BJ2120473
Charles W. Parker, Donald A. Kennerly, Timothy J. Sullivan, Activation of phospholipid metabolism during mediator release from stimulated rat mast cells. Journal of Immunology. ,vol. 122, pp. 152- 159 ,(1979)
M J Berridge, C P Downes, M R Hanley, Lithium amplifies agonist-dependent phosphatidylinositol responses in brain and salivary glands Biochemical Journal. ,vol. 206, pp. 587- 595 ,(1982) , 10.1042/BJ2060587
Robin F. IRVINE, Norma HEMINGTON, Rex M. C. DAWSON, The Calcium-Dependent Phosphatidylinositol-Phosphodiesterase of Rat Brain FEBS Journal. ,vol. 99, pp. 525- 530 ,(1979) , 10.1111/J.1432-1033.1979.TB13284.X
Robert H. Michell, Is phosphatidylinositol really out of the calcium gate Nature. ,vol. 296, pp. 492- 493 ,(1982) , 10.1038/296492A0
S. Cockcroft, J. P. Bennett, B. D. Gomperts, Stimulus–secretion coupling in rabbit neutrophils is not mediated by phosphatidylinositol breakdown Nature. ,vol. 288, pp. 275- 277 ,(1980) , 10.1038/288275A0
Robert Hall Michell, CJ Kirk, Lynne M Jones, CP Downes, Judith A Creba, The stimulation of inositol lipid metabolism that accompanies calcium mobilization in stimulated cells: defined characteristics and unanswered questions. Philosophical Transactions of the Royal Society B. ,vol. 296, pp. 123- 138 ,(1981) , 10.1098/RSTB.1981.0177