Stimulus–secretion coupling in rabbit neutrophils is not mediated by phosphatidylinositol breakdown

摘要: In common with other cells which use intracellular Ca2+ to mediate specific cell function, when rabbit neutrophils are stimulated agonists the rate of metabolism phosphatidylinositol (PI) increases. This is normally measured as incorporation radioactive phosphate or inositol into PI, but these reactions presumed be secondary processes following initial breakdown pre-existing PI diacylglycerol. The labels incorporated during stepwise resynthesis via phosphatidic acid (PA). It has been suggested that in sequence biochemical events, starting binding ligand a receptor, and finally resulting expression cellular activity, an early event immediately directed by activation receptor. could then control increase cytoplasmic dependent on this. Here we report analysis temporal relationship between phospholipid changes stimulation. Our evidence suggests neutrophils, PA labelling both consequences not causes rise Ca2+.