Nicalin and its binding partner Nomo are novel Nodal signaling antagonists
作者： Christof Haffner , Mélanie Frauli , Stephanie Topp , Martin Irmler , Kay Hofmann
关键词: Nodal signaling 、 Lefty 、 Nodal signaling pathway 、 Cell fate determination 、 Biology 、 Ectopic expression 、 Genetics 、 Cell biology 、 NODAL 、 Zebrafish 、 Transforming growth factor beta 、 General Biochemistry, Genetics and Molecular Biology 、 General Immunology and Microbiology 、 General Neuroscience 、 Molecular biology
摘要: Nodals are signaling factors of the transforming growth factor-β (TGFβ) superfamily with a key role in vertebrate development. They control variety cell fate decisions required for establishment embryonic body plan. We have identified two highly conserved transmembrane proteins, Nicalin and Nomo (Nodal modulator, previously known as pM5), novel antagonists Nodal signaling. is distantly related to Nicastrin, component Alzheimer's disease-associated γ-secretase, forms complex Nomo. Ectopic expression both proteins zebrafish embryos causes cyclopia, phenotype that can arise from defect mesendoderm patterning mediated by pathway. Accordingly, downregulation resulted an increase anterior axial development enlarged hatching gland. Inhibition ectopic Lefty was rescued reducing levels. Furthermore, Nodal- well Activin-induced inhibited cell-based reporter assay. Our data demonstrate Nicalin/Nomo antagonizes during mesendodermal zebrafish.
unirioja.es
nature.com
core.ac.uk 
sci-hub.se 参考文章(65)
H. Knoetgen, U. Teichmann, M. Kessel, Head-organizing activities of endodermal tissues in vertebrates. Cellular and Molecular Biology. ,vol. 45, pp. 481- 492 ,(1999)
Patrick Blader, Uwe Strähle, Casting an eye over cyclopia Nature. ,vol. 395, pp. 112- 113 ,(1998) , 10.1038/25836
Dieter Edbauer, Edith Winkler, Joerg T. Regula, Brigitte Pesold, Harald Steiner, Christian Haass, Reconstitution of γ-secretase activity Nature Cell Biology. ,vol. 5, pp. 486- 488 ,(2003) , 10.1038/NCB960
Bernard Thisse, Christopher V. E. Wright, Christine Thisse, Activin- and Nodal-related factors control antero-posterior patterning of the zebrafish embryo. Nature. ,vol. 403, pp. 425- 428 ,(2000) , 10.1038/35000200
Benjamin Feldman, Michael A. Gates, Elizabeth S. Egan, Scott T. Dougan, Gabriela Rennebeck, Howard I. Sirotkin, Alexander F. Schier, William S. Talbot, Zebrafish organizer development and germ-layer formation require nodal-related signals Nature. ,vol. 395, pp. 181- 185 ,(1998) , 10.1038/26013
Alexander F. Schier, Michael M. Shen, Nodal signalling in vertebrate development Nature. ,vol. 403, pp. 385- 389 ,(2000) , 10.1038/35000126
Michael S. Wolfe, Weiming Xia, Beth L. Ostaszewski, Thekla S. Diehl, W. Taylor Kimberly, Dennis J. Selkoe, Two transmembrane aspartates in presenilin-1 required for presenilin endoproteolysis and gamma-secretase activity. Nature. ,vol. 398, pp. 513- 517 ,(1999) , 10.1038/19077
Sangram S. Sisodia, Peter H. St George-Hyslop, γ-Secretase, notch, Aβ and alzheimer's disease: Where do the presenilins fit in? Nature Reviews Neuroscience. ,vol. 3, pp. 281- 290 ,(2002) , 10.1038/NRN785
Gang Yu, Masaki Nishimura, Shigeki Arawaka, Diane Levitan, Lili Zhang, Anurag Tandon, You-Qiang Song, Ekaterina Rogaeva, Fusheng Chen, Toshitaka Kawarai, Agnes Supala, Lyne Levesque, Haung Yu, Dun-Sheng Yang, Erin Holmes, Paul Milman, Yan Liang, Dong Mei Zhang, Dong Hong Xu, Christine Sato, Evgeny Rogaev, Marsha Smith, Christopher Janus, Yanni Zhang, Ruedi Aebersold, Lindsay Farrer, Sandro Sorbi, Amalia Bruni, Paul Fraser, Peter St George-Hyslop, Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and βAPP processing Nature. ,vol. 407, pp. 48- 54 ,(2000) , 10.1038/35024009
M. Furutani-Seiki, C. Nusslein-Volhard, R. N. Kelsh, C.-P. Heisenberg, F. Pelegri, J. Odenthal, M. Granato, D. A. Kane, M. C. Mullins, F. J. M. Van Eeden, R. M. Warga, M. Brand, Y.-J. Jiang, P. Haffter, M. Hammerschmidt, dino and mercedes, two genes regulating dorsal development in the zebrafish embryo Development. ,vol. 123, pp. 95- 102 ,(1996) , 10.1242/DEV.123.1.95