Ethanol-induced modifications to membrane lipid structure: Effect on phospholipase A2-membrane interactions

摘要: Abstract Chronic ethanol intoxication leads to the development of a resistance lipid disordering by ethanol, phenomenon known as “membrane tolerance.” In absence added order, measured ESR and fluorescence techniques, does not necessarily change result chronic ingestion (as in liver microsomes, for example). This suggests that spectroscopic techniques detect tolerance somewhat indirectly, modification responsible may reside region distinct from being probed also raises question whether membrane is associated with an alteration structure. Here we show microsomes rats treated chronically are rendered relatively resistant hydrolytic action exogenous phospholipase A 2 , compared preparations control animals. persists reconstituted vesicles prepared extracted phospholipids. Since same substrate (1-palmitoyl-2- N -(4-nitrobenzo-2-oxa-1,3-diazole)amino caproylphosphatidylcholine) was used both membranes ethanol-treated animals controls, appears structure and/or organization membrane. Further evidence modified treatment provided observation perturbance structural integrity increasing levels oleic acid led progressive loss ethanol-induced relative hydrolysis . The results this study support idea involves probably at bilayer surface. use provides new method probing modifications induced ingestion.