Efficacy of novel recombinant fowlpox vaccine against recent Mexican H7N3 highly pathogenic avian influenza virus.

摘要: Since 2012, H7N3 highly pathogenic avian influenza (HPAI) has produced negative economic and animal welfare impacts on poultry in central Mexico. In the present study, chickens were vaccinated with two different recombinant fowlpox virus vaccines (rFPV-H7/3002 2015 H7 hemagglutinin [HA] gene insert, rFPV-H7/2155 2002 HA insert), then challenged three weeks later HPAI (A/chicken/Jalisco/CPA-37905/2015). The rFPV-H7/3002 vaccine conferred 100% protection against mortality morbidity, significantly reduced shed titers from respiratory gastrointestinal tracts. contrast, of sham birds at higher died within 4 days. Pre- (15/20) post- (20/20) challenge serum had antibodies detectable by hemagglutination inhibition (HI) assay using antigen. However, only a few (3/20) group that reacted strain but all homologous antigen (A/turkey/Virginia/SEP-66/2002) (20/20). One possible explanation for differences efficacy is antigenic drift between circulating viruses vaccines. Molecular analysis demonstrated Mexican strains have continued to rapidly evolve since 2012. addition, we identified silico potential new N-glycosylation sites globular head A/chicken/Jalisco/CPA-37905/2015 virus, which absent 2012 outbreak virus. Our results suggested mutations including increased glycosylation sites, accumulated HPAIV strains, altered recognition neutralizing older rFPV-H7/2155. Therefore, protective novel recent confirms importance frequent updating seed long-term effective control