Immunomarker studies of fine-needle cytopuncture cell blocks for tumor response prediction after preoperative chemotherapy and prognosis in operable nonmetastatic primary breast carcinoma.

摘要: :  Neo-adjuvant chemotherapy of breast cancer provides an opportunity to evaluate predictive factors at initial tumor biopsy. We evaluated these on cell blocks obtained by diagnostic fine-needle cytopuncture (FNC), with respect regression and outcome. A prospective study (1996–2003, median follow-up 82 months) involved 163 patients carcinoma (T2 ≥3 cm, T3, T4 noninflammatory) diagnosed means FNC. Malignancy, cytologic grade, the presence lymphocytes were determined smears. Ki67, estrogen receptor (ER), progesterone (PgR), HER2, p53 expression was assessed immunohistochemistry. All received anthracycline-based chemotherapy. combined clinical pathologic score calculated. Twelve cases (7.5%) showed a complete regression, 72 (44%) partial 79 (48.5%) no regression. Factors high lymphocytes, pN0, Ki67 expression, hormone negativity, “triple negative” phenotype. In univariate analysis 5-year metastasis-free survival rate (MFS) correlated pN, ER, status, while overall (OS) type surgery, ER status. multivariate analysis, MFS significantly influenced score, age, size. Except for same parameters OS. FNC block technique is rapid, minimally invasive, reliable, inexpensive method analyzing biomarkers, may thus be useful in management requiring neo-adjuvant