Profiling Carbonylated Proteins in Heart and Skeletal Muscle Mitochondria from Trained and Untrained Mice
作者： Andrea Carpentieri , Tania Gamberi , Alessandra Modesti , Angela Amoresano , Barbara Colombini
DOI: 10.1021/ACS.JPROTEOME.6B00475
关键词:
摘要: Understanding the relationship between physical exercise, reactive oxygen species, and skeletal muscle modification is important in order to better identify benefits or damages that appropriate inappropriate exercise can induce. Heart muscles have a high density of mitochondria with robust energetic demands, plasticity has an role both cardiovascular system responses. The aim this study was investigate influence regular activity on oxidation profiles mitochondrial proteins from heart tibialis anterior muscles. To end, we used mouse as animal model. Mice were divided into two groups: untrained regularly trained. carbonylated protein pattern studied by two-dimensional gel electrophoresis followed Western blot anti-dinitrophenyl hydrazone antibodies. Mass spectrometry analysis allowed identification several different sites, including methionine, ...
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Maria Fedorova, Nadezhda Kuleva, Ralf Hoffmann, Identification, quantification, and functional aspects of skeletal muscle protein-carbonylation in vivo during acute oxidative stress. Journal of Proteome Research. ,vol. 9, pp. 2516- 2526 ,(2010) , 10.1021/PR901182R
Irina V. Perevoshchikova, Casey L. Quinlan, Adam L. Orr, Akos A. Gerencser, Martin D. Brand, Sites of superoxide and hydrogen peroxide production during fatty acid oxidation in rat skeletal muscle mitochondria Free Radical Biology and Medicine. ,vol. 61, pp. 298- 309 ,(2013) , 10.1016/J.FREERADBIOMED.2013.04.006
Renata L. S. Goncalves, Casey L. Quinlan, Irina V. Perevoshchikova, Martin Hey-Mogensen, Martin D. Brand, Sites of Superoxide and Hydrogen Peroxide Production by Muscle Mitochondria Assessed ex Vivo under Conditions Mimicking Rest and Exercise Journal of Biological Chemistry. ,vol. 290, pp. 209- 227 ,(2015) , 10.1074/JBC.M114.619072
Julie St-Pierre, Julie A. Buckingham, Stephen J. Roebuck, Martin D. Brand, Topology of Superoxide Production from Different Sites in the Mitochondrial Electron Transport Chain Journal of Biological Chemistry. ,vol. 277, pp. 44784- 44790 ,(2002) , 10.1074/JBC.M207217200
Ani Temple, Ten -Yang Yen, Scott Gronert, Identification of specific protein carbonylation sites in model oxidations of human serum albumin. Journal of the American Society for Mass Spectrometry. ,vol. 17, pp. 1172- 1180 ,(2006) , 10.1016/J.JASMS.2006.04.030
Christian Frezza, Sara Cipolat, Luca Scorrano, Organelle isolation: functional mitochondria from mouse liver, muscle and cultured fibroblasts. Nature Protocols. ,vol. 2, pp. 287- 295 ,(2007) , 10.1038/NPROT.2006.478
S. Bayod, J. del Valle, J.F. Lalanza, S. Sanchez-Roige, B. de Luxán-Delgado, A. Coto-Montes, A.M. Canudas, A. Camins, R.M. Escorihuela, M. Pallàs, Long-term physical exercise induces changes in sirtuin 1 pathway and oxidative parameters in adult rat tissues Experimental Gerontology. ,vol. 47, pp. 925- 935 ,(2012) , 10.1016/J.EXGER.2012.08.004
Taku Hamada, Edward B. Arias, Gregory D. Cartee, Increased submaximal insulin-stimulated glucose uptake in mouse skeletal muscle after treadmill exercise Journal of Applied Physiology. ,vol. 101, pp. 1368- 1376 ,(2006) , 10.1152/JAPPLPHYSIOL.00416.2006
Scott K. Powers, Malcolm J. Jackson, EXERCISE-INDUCED OXIDATIVE STRESS: CELLULAR MECHANISMS AND IMPACT ON MUSCLE FORCE PRODUCTION Physiological Reviews. ,vol. 88, pp. 1243- 1276 ,(2008) , 10.1152/PHYSREV.00031.2007
Richard J. Bloomer, Andrew C. Fry, Michael J. Falvo, Christopher A. Moore, Protein carbonyls are acutely elevated following single set anaerobic exercise in resistance trained men. Journal of Science and Medicine in Sport. ,vol. 10, pp. 411- 417 ,(2007) , 10.1016/J.JSAMS.2006.07.014