Molecular targets of antimicrobial photodynamic therapy identified by a proteomic approach.

摘要: Antimicrobial photodynamic therapy (PDT) is a promising tool to combat antibiotic-resistant bacterial infections. During PDT, bacteria are killed by reactive oxygen species generated visible light absorbing photosensitizer (PS). We used classical proteomic approach that included two-dimensional gel electrophoresis and mass spectrometry analysis, identify some proteins of Staphylococcus aureus damaged during PDT with the cationic PS meso-tetra-4-N-methyl pyridyl porphine (T4). Suspensions S. cells were incubated selected T4 concentrations irradiated doses blue reduced survival about 60% or 1%. Proteomics analyses membrane enriched fraction revealed these sub-lethal treatments affected expression several functional classes proteins, this damage selective. Most found be involved in metabolic activities, oxidative stress response, cell division uptake sugar. Subsequent delayed growth considerably glucose consumption capacity cells. This investigation provides new insights towards characterization induced mechanism killing using, for first time, approach.