Mesenchymal to embryonic incomplete transition of human cells by chimeric OCT4/3 (POU5F1) with physiological co-activator EWS.
作者： Hatsune Makino , Masashi Toyoda , Kenji Matsumoto , Hirohisa Saito , Koichiro Nishino
DOI: 10.1016/J.YEXCR.2009.06.016
关键词:
摘要: POU5F1 (more commonly known as OCT4/3) is one of the stem cell markers, and affects direction differentiation in embryonic cells. To investigate whether cells mesenchymal origin acquire phenotypes, we generated human mesodermal with overexpression chimeric OCT4/3 gene physiological co-activator EWS (product EWSR1 gene), which driven by potent promoter translocation. The expressed genes such NANOG, lost exhibited embryonal cell-like alveolar structures when implanted into subcutaneous tissue immunodeficient mice. Hierarchical analysis microchip surface revealed that are subcategorized group carcinoma These results imply can be traced back to phenotype collaboration cis-regulatory element fused co-activator. this study provide us insight plasticity involving essential for maintenance, complexity required changing cellular identity.
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