Study of Bacillus subtilis Endospores in Soil by Use of a Modified Endospore Stain.

摘要: The Schaeffer-Fulton endospore stain was modified so that it would Bacillus subtilis endospores in soil smears. differentiated among dormant spores, spores undergoing activation, and which had germinated but not yet shown outgrowth. These differentiations were seen for pure spore preparations the laboratory. This used to show reversible B. activation promoted by an Ensifer adhaerens-like indigenous bacterium cultures of E. adhaerens added Under specific conditions laboratory, germination did proceed beyond stage, relatively little change occurred numbers both subtilis. also true soil, although some with destruction vegetative cells occur if been nutritionally enriched preincubation incorporated ground alfalfa.