A general method for the purification of synthetic oligodeoxyribonucleotides containing strong secondary structure by reversed-phase high-performance liquid chromatography on PRP-1 resin.
作者： Markus W. Germann , Richard T. Pon , Johan H. van de Sande
DOI: 10.1016/0003-2697(87)90288-0
关键词:
摘要: Synthetic 5'-dimethoxytritylated oligodeoxyribonucleotides, which contained strong secondary structure, were satisfactorily denatured and purified by reversed-phase HPLC on PRP-1 columns when strongly alkaline conditions (0.05 M NaOH) employed. This procedure was suitable for the purification of hairpin structures, e.g., d(CG)nT4(CG)n (n = 4, 5, 6), oligo(dG) sequences, d(G)24, as well oligodeoxyribonucleotide probes degenerate base sites. Oligodeoxyribonucleotides long 50 bases in length purified. Recovery injected oligonucleotides typically 90% or better. The high capacity resin also allowed to be performed a preparative scale (2-8 mg per injection). Enzymatic degradation analysis indicated that no modification heterocyclic occurred under described.
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