Analysis and Purification of Synthetic Nucleic Acids Using HPLC
作者： Alex Andrus , Robert G. Kuimelis
DOI: 10.1002/0471142700.NC1005S01
关键词:
摘要: HPLC is a powerful and popular method for analyzing purifying biomolecules. Reversed-phase allows high-capacity purification, uses volatile buffer systems that simplify product recovery. Anion-exchange provides better resolution more predictable elution pattern. This unit presents protocols are optimized of oligonucleotides. Because the limits both reversed-phase anion-exchange HPLC, it can be used oligonucleotides up to approximately 50 nt in length.
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