Separation of synthetic phosphorothioate oligodeoxynucleotides from their oxygenated (phosphodiester) defect species by strong-anion-exchange high-performance liquid chromatography
作者： B.John Bergot , W. Egan
DOI: 10.1016/0021-9673(92)85456-4
关键词:
摘要: Abstract A method is described which separates synthetic deoxynucleotide phosphorothioates from their oxygenated (phosphodiester, “P = O”) defect species by strong-anion-exchange chromatography, using novel “soft-base” anionic eluents. The enables the qualitative and quantitative assessment of P O content in phosphorothioate DNA, represents a rapid sample-conserving alternative to 31 P-NMR.
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