Two-dimensional electrophoresis of protein antigens with an antibody containing buffer

摘要: Abstract A method of protein analysis is described which involves a first migration in starch gel, followed by second stabilized buffer, contains dissolved antiserum. By conducting the at right angles to first, overlap components complex solutions was avoided. Serum fractions were analyzed separately, and together combined solution. Identification solution made absorption technics. When applied human serum, large number lines or zones precipitation demonstrated. Absorption addition studies with above indicate that most these represent electrophoretically immunologically distinct serum proteins.