Rapid identification of Trichophyton tonsurans by PCR-RFLP analysis of ribosomal DNA regions

摘要: Background: Culture morphology of Trichophyton (T.) tonsurans, an emerging pathogen dermatophytosis in Japan, varies widely and species level identification is sometimes very difficult. Reliable molecular markers are expected to be introduced for their identification. Objective: The present study was conducted evaluate the efficacy restriction fragment length polymorphism (RFLP) analysis PCR amplified ribosomal (r) DNA including internal transcribed spacers (ITS), as tool. Methods: Total cellular extracted from 26 Japanese isolates T. along with several taxa members mentagrophytes complex, rubrum, violaceum Epidermophyton floccosum, using a mini-preparation method. amplicons were digested enzymes Mva I or Hinf I, then electrophoresed on 5% polyacrylamide gel. Results: banding profiles observed about 8 h initiating extraction. Intraspecies not detected among tonsurans isolates, obtained digestion clearly different those other dermatophyte species. evaluated nucleotide sequence regions by computer compatible Conclusion: PCR-RFLP rapid reliable tool tonsurans.