Enzymatic synthesis of radioactive (−)-carnitine from γ-butyrobetaine prepared by the methylation of γ-aminobutyric acid
作者： Albert Daveluy , Rehana Parvin , Shri V. Pande
DOI: 10.1016/0003-2697(82)90587-5
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摘要: Abstract Radioactive γ-butyrobetaine was prepared by quaternization of γ-aminobutyric acid with tritiated methyl iodide under conditions giving high yields respect to both the above precursors. Part product passed through a column ion-retardation resin and gave radioactive good purity. The remainder converted (−)-carnitine stoichiometrically employing 50–60% ammonium sulfate fraction rat liver supernatant as source hydroxylase (EC 1.14.11.1). Successive chromatographies on cation exchanger resins then purity in yield.
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