Using CD133 positive U251 glioblastoma stem cells to establish nude mice model of transplanted tumor.
作者： Feng Jin , Chao Gao , Lei Zhao , Hao Zhang , Hai-Tao Wang
DOI: 10.1016/J.BRAINRES.2010.10.051
关键词:
摘要: This study tried to use CD133 positive U251 glioblastoma stem cells establish nude mice model of transplanted tumor. were isolated and identified. Twenty-five male divided into three groups: cell group, group normal control group. respectively inoculated mouse brain Mice growing condition behavior observed. Magnetic resonance imaging (MRI) was performed detect tumor growth in brain. HE staining assay, immunohistochemical assay CD34 marked microvascular density (MVD) test performed. As a result, the successful rates both groups 100%. Growing more significantly exaggerated image magnetic malignant than that It could be found models showed stronger pathogolical malignance features Glial fibrillary acidic protein (GFAP) expression originated from astrocytes. highest higher other two groups. In conclusion, is an excellent method likely one established by cancer cells, which new animal research glioma.
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