Role of plasminogen activator and of 92-KDa type IV collagenase in glioblastoma invasion using an in vitro matrigel model

摘要: The invasive nature of human gliomas represents a major factor in preventing their total resection. exact the underlying mechanisms tumor cell invasion are still unclear. In this study, we have quantitatively assayed glioblastoma line for its ability to migrate through polycarbonate filter coated with matrigel which contains complex multiple basement membrane components. At 48 h (U251) showed rate invasiveness 42% and also dependent on concentration matrigel. U251 produced urokinase type plasminogen activator 92-KDa IV collagenase. Both enzymes were inhibited by addition uPA collagenase antibodies. Those same antibodies reduced cells from 12 21%, respectively. Similarly, ɛ-aminocaproic acid (a plasmin inhibitor) or tissue inhibitor metalloprotease (TIMP2, 14% 10%, Additionally, other two lines (LG11, UWR1) astrocytes at 41%, 61% 12%, Finally, hyaluronic matrigel, constituent brain extracellular matrix, enhanced invasion. These findings provide evidence role serine proteases metalloproteases facilitating matrix components suggest therapeutic protease inhibitors attempting minimize propensity gliomas.