[16] Human factor XII (hageman factor)

摘要: Publisher Summary In this chapter, a purification method of human factor XII is described that reproducible and suitable for obtaining milligram amounts protein. For frozen outdated plasma used, including following procedures: ammonium sulfate fractionation; first DEAE-Sephadex column chromatography; second QAE-Sephadex CM-celhdose separation Factor by Benzamidine-Agarose chromatography. The clotting activity determined kaolin partial thromboplastin time using XII-deficient plasma. continuous tilting the tubes at 37°C. units are calculated from standard calibration curve prepared serial dilution pooled normal Amidase measured synthetic peptide substrate D-prolyl-L-phenylalanyl-L-arginine-p-nitroaniline.