Production and characterization of a murine monoclonal antibody against a heavy chain of Hageman factor (factor XII).

摘要: A murine hybridoma cell line that produces a monoclonal antibody to human Hageman factor (HF, XII) is described. The (P 5–2– 1) consists of mouse IgG2b heavy chains and lambda light chains, selectively neutralizes HF procoagulant activity, prevents the proteolytic cleavage during contact activation in plasma. When exposed P 5–2–1 before absorption kaolin, activity markedly inhibited. In contrast, does not interfere with after adsorption kaolin. inactivate prekallikrein–activating 28,000–mol wt fragments (HFf). binds exclusively 40,000mol portion chain inhibits negatively charged surfaces. immobilized on Sepharose can be used deplete from normal This immunoaffinity-depleted plasma indistinguishable congenital HF- deficient as substrate for assay.