Mycoplasma hyorhinis molecules that induce tumor necrosis factor alpha secretion by human monocytes.

摘要: Mycoplasma hyorhinis has been shown to induce the secretion of tumor necrosis factor alpha (TNF-alpha) from monocytes. To identify molecules responsible for this activity, we separated sonicated M. lysate material by centrifugation at 100,000 x g into soluble (S) and particulate (P) fractions. The fractions were assayed TNF-alpha-inducing activity L929 bioassay. Both able TNF-alpha in roughly equal amounts. optimum dose both was 1 micrograms/ml. Proteinase K treatment either fraction eliminated suggesting that a protein component is involved induction. Phase partitioning Triton X-114 aqueous (A) detergent (D) phases showed composed 80% aqueous-phase proteins, while > 75% detergent-phase proteins. All four (SA, SD, PA, PD) release. Treatment with NaIO4 remove carbohydrate reduced inducing SA phase 80%, whereas other unaffected treatment. M(r)S determined monocyte Western (immunoblot) technique. associated single periodate-sensitive peak 69 75 kDa. two had similar profiles containing peaks activity. These corresponded 48 52, 43 45, 39 40, 31 32 PA also contained 75, 55 57, 40 Thus, glycan moiety are capable release human