Vascular endothelial growth factor, interleukin 8, platelet-derived endothelial cell growth factor, and basic fibroblast growth factor promote angiogenesis and metastasis in human melanoma xenografts.

摘要: Angiogenesis is a significant prognostic factor in melanoma, but the angiogenic factors controlling neovascularization are not well defined. The purpose of this study was to investigate whether angiogenesis and metastasis melanoma promoted by vascular endothelial growth (VEGF), interleukin 8 (IL-8), platelet-derived cell (PD-ECGF), and/or basic fibroblast (bFGF). Cells from human lines (A-07, D-12, R-18, U-25) transplanted BALB/c nu/nu mice were used as tumor models. Expression studied ELISA, Western blotting, immunohistochemistry. assessed using an intradermal assay. Lung colonization spontaneous lung determined after i.v. inoculation cells, respectively. specific roles VEGF, IL-8, PD-ECGF, bFGF angiogenesis, colonization, treated with neutralizing antibody. expressed multiple factors, each line showed unique expression pattern. Multiple most lines, whereas least possibly solely VEGF. Tumor growth, controlled rate hence promoting angiogenesis. A-07 inhibited treatment antibody against or bFGF. Each these may promote because inhibition one them could be compensated for others. Our observations suggest that efficient antiangiogenic require identification blocking common functional features several factors.