UV-ozone ashing of cells and tissues for spatially resolved trace element analysis
作者： B Gilbert , L Perfetti , R Hansen , D Mercanti , P Casalbore
DOI: 10.2741/A488
关键词:
摘要: UV/ozone ashing of thin tissue sections and cell cultures is a simple technique to enhance relative elemental concentrations, while maintaining their spatial location at the sub-micron level. This approach may capability spatially resolved analysis techniques detect distribution trace elements in biological matrices. We present results from light microscopy x-ray spectromicroscopy studies tissues cells demonstrating that micro-structure very well conserved. show signal enhancement resulting removal carbon, which allows otherwise undetectable gadolinium be mapped cancer for novel neutron capture therapy.
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