Spatial Regionalization and Heterochrony in the Formation of Adult Pallial Neural Stem Cells

摘要: Little is known on the embryonic origin and related heterogeneity of adult neural stem cells (aNSCs). We use conditional genetic tracing, activated in a global or mosaic fashion by cell type-specific promoters focal laser uncaging, coupled with gene expression analyses Notch invalidations, to address this issue zebrafish telencephalon. report that germinal zone pallium originates from two distinct subtypes progenitors integrates modes aNSC formation. Dorsomedial aNSCs derive amplification actively neurogenic radial glia On contrary, lateral population formed stepwise addition at pallial edge discrete neuroepithelial progenitor pool posterior telencephalic roof, postembryonic stages persisting lifelong. This dual allows temporally organized building territories as patchwork juxtaposed compartments.