In vivo contribution of nestin- and GLAST-lineage cells to adult hippocampal neurogenesis.

摘要: Radial glia-like cells (RGCs) are the hypothesized source of adult hippocampal neurogenesis. However, current model neurogenesis does not fully incorporate in vivo heterogeneity RGCs. In order to better understand contribution different RGC subtypes neurogenesis, we employed widely used transgenic lines (Nestin-CreER(T2) and GLAST::CreER(T2) mice) explore how RGCs contribute under basal conditions after stimulation depletion neural progenitor cells. We first these inducible fate-tracking define similarities differences nestin- GLAST-lineage long-term then explored ability experimental manipulations that either ablate (i.c.v. application anti-mitotic AraC, cytosine-β-D-arabinofuranoside) or stimulate (wheel running). Interestingly, both ablation experiments, labeled mice appear whereas Nestin-CreER(T2) do not. Finally, using NestinGFP reporter mice, expanded on previous research by showing all dentate gyrus subgranular zone express nestin, therefore antigenically heterogeneous. These findings important for field, as they allow appropriately conservative interpretation existing future data emerge from lines. also raise questions about between driver lines, RGCs, potential cell behavior As highlight possible vivo, indicate models should be modified include lineage heterogeneity.