Immobilization of native and dextran-free dextransucrases from Leuconostoc mesenteroides NRRL B-512F for the synthesis of glucooligosaccharides
作者： M. Alcalde , F.J. Plou , A. Gómez de Segura , M. Remaud-Simeon , R.M. Willemot
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摘要: Dextransucrase from Leuconostoc mesenteroides NRRL B-512F was immobilized using two different methods: covalent attachment to activated silica and entrapment in calcium alginate. For immobilization on silica, native enzyme dextran-free were compared. However, the alginate beads gave best results terms of yield stability. This biocatalyst employed acceptor reaction with maltose showing similar glucooligosaccharide production than but increased operational
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