Modification of Membrane Function, Protein Synthesis, and Heat Killing Effect in Cultured Chinese Hamster Cells by Glycerol and D2O

摘要: Abstract Glycerol and D2O can provide a protective effect to Chinese hamster V79 cells receiving heat treatment. The mechanism for this is assumed be the ability of these agents stabilize proteins which implies that one possible heat-killing mechanisms inactivation heat-sensitive molecule (protein?). In addition, we observed alter membrane permeability rapidly, glycerol reduce initial heat-induced changes (determined by aminoisobutyric acid uptake). Although not as effective cycloheximide, retard protein synthesis. These two processes add stabilizing cellular against killing. Since added during heat-conditioning period interfere with appearance thermotolerance, are likely different from thermotolerance. We propose protected or may also play role in triggering process thermotolerance phenomenon. If conditioning treatment sufficient affect but other targets, thus allowing cell survive, surviving response second continued Depending on severity effect, continue exist after medium containing D2O, therefore, challenging altered little. This proposed capable explaining several experimental protocols. toxicity vincristine, microtubule-related probably stabilized agent. affects microtubule protein, because heated show little topological changes.