Evaluation of a high-affinity QCM immunosensor using antibody fragmentation and 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer.
作者： Shigeru Kurosawa , Miki Nakamura , Jong-Won Park , Hidenobu Aizawa , Kazunori Yamada
DOI: 10.1016/J.BIOS.2004.05.016
关键词:
摘要: This study evaluated construction of a highly affinitive quartz crystal microbalance (QCM) immunosensor using anti-C-reactive protein (CRP) antibody and its fragments for CRP detection. Three types were immobilized on the surface QCM via covalent-bounding. Then affinity was through antigen-antibody binding between antibody. Affinity shown to be highest when anti-CRP F(ab')2-IgG (70 microg/mL) QCM. In case antibody, which attributable almost twice that IgG is used conventionally immunosensors. addition, it treated with 2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate, so-called MPC polymer, selective immunosensing achieved without non-specific from plasma proteins in human serum. When QCM, detection limit linearity calibration curve at concentrations 0.001 100 microg/dL even during investigation serum samples. Experimental results verified successful QCM-immunosensor modified polymer.
elsevier.com
sci-hub.se 参考文章(29)
J Lüdemann, W G Wood, J Heinrich, U Frick, R Mitusch, R Maass, Evaluation of a sensitive immunoluminometric assay for the determination of C-reactive protein (CRP) in serum and plasma and the establishment of reference ranges for different groups of subjects. Clinical Laboratory. ,vol. 46, pp. 131- 140 ,(2000)
C L Morgan, D J Newman, C P Price, Immunosensors: technology and opportunities in laboratory medicine Clinical Chemistry. ,vol. 42, pp. 193- 209 ,(1996) , 10.1093/CLINCHEM/42.2.193
Luis Borque, Laura Bellod, Antonio Rus, M Luisa Seco, Francisco Galisteo-González, Development and validation of an automated and ultrasensitive immunoturbidimetric assay for C-reactive protein. Clinical Chemistry. ,vol. 46, pp. 1839- 1842 ,(2000) , 10.1093/CLINCHEM/46.11.1839
J. M. Abad, F. Pariente, L. Hernández, H. D. Abruña, E. Lorenzo, Determination of Organophosphorus and Carbamate Pesticides Using a Piezoelectric Biosensor Analytical Chemistry. ,vol. 70, pp. 2848- 2855 ,(1998) , 10.1021/AC971374M
Wolfgang Koenig, Malte Sund, Margit Fröhlich, Hans-Günther Fischer, Hannelore Löwel, Angela Döring, Winston L. Hutchinson, Mark B. Pepys, C-Reactive Protein, a Sensitive Marker of Inflammation, Predicts Future Risk of Coronary Heart Disease in Initially Healthy Middle-Aged Men Results From the MONICA (Monitoring Trends and Determinants in Cardiovascular Disease) Augsburg Cohort Study, 1984 to 1992 Circulation. ,vol. 99, pp. 237- 242 ,(1999) , 10.1161/01.CIR.99.2.237
Shigeru Kurosawa, Hidenobu Aizawa, Mitsuhiro Tozuka, Miki Nakamura, Jong-Won Park, Immunosensors using a quartz crystal microbalance Measurement Science and Technology. ,vol. 14, pp. 1882- 1887 ,(2003) , 10.1088/0957-0233/14/11/005
Michael. Thompson, Catherine L. Arthur, Gurbaksh K. Dhaliwal, Liquid-phase piezoelectric and acoustic transmission studies of interfacial immunochemistry. Analytical Chemistry. ,vol. 58, pp. 1206- 1209 ,(1986) , 10.1021/AC00297A051
Makoto. Muratsugu, Fumihiko. Ohta, Yoshihiro. Miya, Toshiaki. Hosokawa, Shigeru. Kurosawa, Naoki. Kamo, Hisami. Ikeda, Quartz crystal microbalance for the detection of microgram quantities of human serum albumin: relationship between the frequency change and the mass of protein adsorbed Analytical Chemistry. ,vol. 65, pp. 2933- 2937 ,(1993) , 10.1021/AC00068A036
K. Ishihara, Bioinspired phospholipid polymer biomaterials for making high performance artificial organs Science and Technology of Advanced Materials. ,vol. 1, pp. 131- 138 ,(2000) , 10.1016/S1468-6996(00)00012-7
Thomas B Ledue, Debra L Weiner, Jean D Sipe, Sue E Poulin, Marilyn F Collins, Nader Rifai, None, Analytical evaluation of particle-enhanced immunonephelometric assays for C-reactive protein, serum amyloid A and mannose-binding protein in human serum. Annals of Clinical Biochemistry. ,vol. 35, pp. 745- 753 ,(1998) , 10.1177/000456329803500607