Apical membranes prepared by peeling from whole porcine oviducts interact with homologous sperm
作者： Juan M. Teijeiro , Patricia E. Marini
DOI: 10.1007/S00441-012-1338-3
关键词:
摘要: In mammals, interaction between sperm and oviductal epithelial cells provides the formation of a reservoir selection at isthmus oviduct. Several in vitro methods are used to study this interaction. Apical plasma membranes (APM) have been prepared by peeling from culture differentiated kidney cells. work, we modify method, using it for preparation APM directly whole oviduct, proving purity apical obtained western blot proteins known specific locations. The correspond only most cells, exposed lumen organ. Also, shown biotinylation interact with sperm. binding is head induces on them prolonged motility tyrosine phosphorylation masses 92, 97, 210 220 kDa. p97 has previously described as an effect membrane glycoprotein (SBG), which be present preparations here. Upon treatment APM, pattern changes heads tail. Thus, describe easy method organs that allows their context permits sperm–oviduct studies. This renders specifically located
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