Localization and developmental expression patterns of CSPG-cs56 (aggrecan) in normal and dystrophic retinas in two rat strains.

摘要: Abstract Proteoglycans have a number of important functions in the central nervous system. Aggrecan (hyaluronan-binding proteoglycan, CSPG-cs56) is found extracellular matrix cartilage as well developing brain. We compared postnatal distribution CSPG-cs56 Long Evans (LE) and Royal College Surgeons (RCS) rat retinas to determine if this proteoglycan played role development dystrophic retinas. expression was examined aged between birth (postnatal day 0, P0) P150 using immunofluorescence Western-blots. Immunofluorescence quantified ImageJ. GFAP staining used compare Muller cell labeling CSPG-cs56. Both strains showed significant rise total retinal P0 P21; values peaked on P21 LE rats P14 RCS rats. then significantly decreased lower levels (P35) both before reaching higher by P90–P150. positive present ganglion layer at clear layering inner plexiform seen P7 due dendritic cells. Staining less intense diffuse within outer over similar time-course. Light region segments (P14) became more retina approached maturity. main contributor older animals. Substantial differences were not There no evidence suggest that cells source either strain, although their distributions had degree overlap. The lack indicates may be involved degenerative process or reorganization retina. CPSG-cs56 maintain structure closely related providing adequate support flexibility for photoreceptor segments, which necessary function.