Glutaminolysis drives membrane trafficking to promote invasiveness of breast cancer cells.

摘要: The role of glutaminolysis in providing metabolites to support tumour growth is well-established, but the involvement glutamine metabolism invasive processes yet be elucidated. Here we show that normal mammary epithelial cells consume glutamine, do not secrete glutamate. Indeed, low levels extracellular glutamate are necessary maintain homoeostasis, and provision drives disruption morphology promotes key characteristics phenotype such as lumen-filling basement membrane disruption. By contrast, primary cultures breast cancer convert which released from cell through system Xc- antiporter activate a metabotropic receptor. This contributes intrinsic aggressiveness these by upregulating Rab27-dependent recycling transmembrane matrix metalloprotease, MT1-MMP promote behaviour leading These data indicate acquisition ability release watershed disease aggressiveness.