Automated rapid iterative negative geotaxis assay and its use in a genetic screen for modifiers of Aβ(42)-induced locomotor decline in Drosophila.
作者： Haiyan Liu , Meng Han , Qingyi Li , Xiao Zhang , Wen-An Wang
DOI: 10.1007/S12264-014-1526-0
关键词:
摘要: The negative-geotaxis climbing assay is used to efficiently study aging and neurodegeneration in Drosophila. To make it suitable for large-scale study, a method called the rapid iterative negative geotaxis (RING) has been established by simultaneously photographing of multiple groups flies when they are manually tapped down test tubes. Here, we automated using well-controlled electric motor drive tapping, homemade program analyze height flies. Using RING (aRING) assay, found that ability strain wild-type flies, males particular, declined rapidly before day 21 after eclosion, but slowly from 35. We also expression arctic mutant Aβ42 accelerated age-dependent decline Moreover, aRING, examined effect third chromosome deficiencies on locomotor Aβ42-expressing isolated 7 suppressors 15 enhancers.
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irgrid.ac.cn参考文章(12)
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