Anti-inflammatory effects of an oxylipin-containing lyophilised biomass from a microalga in a murine recurrent colitis model.

摘要: Diet and nutritional factors have emerged as possible interventions for inflammatory bowel diseases (IBD), which are characterised by chronic uncontrolled inflammation of the intestinal mucosa. Microalgal species a promising source n-3 PUFA derived oxylipins, lipid mediators with key role in resolution inflammation. The aim present study was to investigate effects an oxylipin-containing lyophilised biomass from Chlamydomonas debaryana on recurrent 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis mice model. Moderate colon induced BALB/c weekly intracolonic instillations low dose TNBS. Administration microalgal started 2 weeks before induction continued throughout development. Mice were killed 48 h after last TNBS challenge. Oral administration reduced TNBS-induced inflammation, evidenced inhibition body weight loss, improvement morphology decrease pro-inflammatory cytokines TNF-α, IL-1β, IL-6 IL-17. This product also down-regulated colonic expressions inducible nitric oxide, cyclo-oxygenase NF-κB, well increased PPAR-γ. In addition, up-regulated antioxidant transcription factor nuclear E2-related target gene heme oxygenase 1. describes first time prophylactic microalgae C. acute phase model mice. These findings suggest potential use this microalga, or nutraceutical treatment IBD.