A nuclear encoded tRNA of Trypanosoma brucei is imported into mitochondria.
作者： N Agabian , A Schneider , J Martin
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摘要: Abstract The mitochondrial genome of trypanosomes, unlike that most other eukaryotes, does not appear to encode any tRNAs. Therefore, tRNAs must be either imported into the organelle or created through a novel process, such as RNA editing. Trypanosomal tRNA(Tyr), whose gene contains an 11-nucleotide intron, is present in both cytosol and mitochondrion encoded by single-copy nuclear gene. By site-directed mutagenesis, point mutations were introduced this tRNA gene, mutated was reintroduced trypanosomal DNA transfection. Expression mutant led accumulation unspliced tRNA(Tyr) (A. Schneider, K. P. McNally, N. Agabian, J. Biol. Chem. 268:21868-21874, 1993). Cell fractionation revealed significant portion recovered fraction resistant micrococcal nuclease treatment intact organelle. integrated, recovery its product directly demonstrated import. In vitro experiments showed contrast spliced wild-type form, no longer substrate for cognate aminoacyl synthetase. The presence uncharged mitochondria aminoacylation coupled
sci-hub.se 参考文章(22)
P.K. Patnaik, S.K. Kulkarni, G.A. Cross, Autonomously replicating single-copy episomes in Trypanosoma brucei show unusual stability. The EMBO Journal. ,vol. 12, pp. 2529- 2538 ,(1993) , 10.1002/J.1460-2075.1993.TB05908.X
I. Small, L. Maréchal-Drouard, J. Masson, G. Pelletier, A. Cosset, J.H. Weil, A. Dietrich, In vivo import of a normal or mutagenized heterologous transfer RNA into the mitochondria of transgenic plants: towards novel ways of influencing mitochondrial gene expression? The EMBO Journal. ,vol. 11, pp. 1291- 1296 ,(1992) , 10.1002/J.1460-2075.1992.TB05172.X
K Hancock, A.J. LeBlanc, D Donze, S.L. Hajduk, Identification of nuclear encoded precursor tRNAs within the mitochondrion of Trypanosoma brucei. Journal of Biological Chemistry. ,vol. 267, pp. 23963- 23971 ,(1992) , 10.1016/S0021-9258(18)35931-3
S L Hajduk, K Hancock, The mitochondrial tRNAs of Trypanosoma brucei are nuclear encoded. Journal of Biological Chemistry. ,vol. 265, pp. 19208- 19215 ,(1990) , 10.1016/S0021-9258(17)30645-2
M E Harris, D R Moore, S L Hajduk, Addition of uridines to edited RNAs in trypanosome mitochondria occurs independently of transcription. Journal of Biological Chemistry. ,vol. 265, pp. 11368- 11376 ,(1990) , 10.1016/S0021-9258(19)38602-8
J.C. Mottram, S.D. Bell, R.G. Nelson, J.D. Barry, tRNAs of Trypanosoma brucei. Unusual gene organization and mitochondrial importation. Journal of Biological Chemistry. ,vol. 266, pp. 18313- 18317 ,(1991) , 10.1016/S0021-9258(18)55271-6
A Schneider, K.P. McNally, N Agabian, Splicing and 3'-processing of the tyrosine tRNA of Trypanosoma brucei. Journal of Biological Chemistry. ,vol. 268, pp. 21868- 21874 ,(1993) , 10.1016/S0021-9258(20)80621-8
P. Guillemaut, A. Steinmetz, G. Burkard, J.H. Weil, Aminoacylation of tRNALeu species from Escherichia coli and from the cytoplasm, chloroplasts and mitochondria of Phaseolus vulgaris by homologous and heterologous enzymes Biochimica et Biophysica Acta. ,vol. 378, pp. 64- 72 ,(1975) , 10.1016/0005-2787(75)90137-9
Yoshitaka Suyama, Jin Hamada, The mitochondrial and cytoplasmic valyl tRNA synthetases in Tetrahymena are indistinguishable Archives of Biochemistry and Biophysics. ,vol. 191, pp. 437- 443 ,(1978) , 10.1016/0003-9861(78)90382-X
Anneloor L. M. A. ten Asbroek, Marc Ouellette, Piet Borst, Targeted insertion of the neomycin phosphotransferase gene into the tubulin gene cluster of Trypanosoma brucei Nature. ,vol. 348, pp. 174- 175 ,(1990) , 10.1038/348174A0