Muscarinic cholinergic receptors in dorsal root ganglia of chick embryo: a radioligand binding and immunocytochemical study
作者： Ada Maria Tata , Stefano Biagioni , Alberto Ricci , Francesco Amenta , Gabriella Augusti-Tocco
DOI: 10.1016/0304-3940(95)11474-B
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摘要: The presence and micro-anatomical localization of muscarinic cholinergic receptors were assessed in dorsal root ganglia chick embryo during development using radioligand binding immunocytochemical techniques, respectively. non-selective receptor [3H]quinuclidinyl benzilate was specifically bound to sections with a dissociation constant value (Kd) 0.75 +/- 0.02 nM maximum density sites (Bmax) 7.2 0.5 fmol/mg tissue. [3H]Quinuclidinyl partially sensitive pirenzepine displacement. This suggests that expressed by at least part belong the M1 subtype. Immunocytochemical analysis confirmed ganglia. These findings suggest neurons ganglia, which are known express markers such as choline acetyltransferase, acetylcholinesterase high affinity uptake, also cholinoceptive.
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