MIP-1alpha as a critical macrophage chemoattractant in murine wound repair.

摘要: At sites of injury, macrophages secrete growth factors and proteins that promote tissue repair. While this central role the macrophage has been well studied, specific stimuli recruit into injury are not understood. This study examines inflammatory protein 1alpha (MIP-1alpha), a C-C chemokine with monocyte chemoattractant capability, in excisional wound Both MIP-1alpha mRNA were detectable murine wounds from 12 h through 5 d after injury. levels peaked 3 coinciding maximum infiltration. The contribution to recruitment was assessed by treating mice neutralizing anti-MIP-1alpha antiserum before Wounds treated had significantly fewer than control (41% decrease, P < 0. 01). decrease paralleled decreased angiogenic activity collagen synthesis. When tested corneal micropocket assay, homogenates contained less (27% positive for versus 91% group, 0.01). Collagen production also reduced animals (29% 0.05). results demonstrate plays critical wounds, suggest appropriate repair is dependent upon recruitment.