Grafting and early expression of growth factors from adipose-derived stem cells transplanted into the cochlea, in a Guinea pig model of acoustic trauma

摘要: Noise exposure causes damage of multiple cochlear cell types producing permanent hearing loss with important social consequences. In mammals, no regeneration either damaged hair cells or auditory neurons has been observed and successful treatment is available to achieve a functional recovery. Several evidences indicate adipose-derived stem (ASCs) as promising tools in diversified regenerative medicine applications, due the high degree plasticity trophic features. This study was aimed at identifying path vivo migration expression growth factors, upon ASC transplantation into cochlea, following noise-induced injury. ASCs were isolated primary culture from adipose tissue guinea pig, transduced using viral vector express green fluorescent protein, implanted scala tympani deafened animals. Auditory function assessed 3 7 days after surgery. The factors comparatively analyzed real time PCR control noise-injured tissues. Immunofluorescence used assess localization cochleae, homologous implantation. implantation did not modify function. migrated perilymphatic endolymphatic compartment, during course. Upon noise exposure, chemokine ligands receptors related PDGF, VEGF TGFbeta pathways, increased tissues, possibly guiding migration. confirmed expression, which appeared be further strengthened by These results that are able migrate site intracochlear implantantion, providing an original proof principle, could pave way for developments ASC-based treatments deafness.