Fluorescence energy transfer in the bi-fluorescent S-layer tandem fusion protein ECFP―SgsE―YFP

摘要: This work reports for the first time on fabrication of a bi-functional S-layer tandem fusion protein which is able to self-assemble solid supports without losing its functionality. Two variants green fluorescent (GFP) were genetically combined with self-assembly system having remarkable opportunity interact each other and act as functional nanopatterning biocoating. The SgsE Geobacillus stearothermophilus NRS 2004/3a was fused cyan ECFP donor at N-terminus yellow YFP acceptor C-terminus. fluorescence energy transfer studied spectrofluorimetry, confocal microscopy flow cytometry, whilst (on silicon dioxide particles) structural investigations carried out atomic force (AFM). resonance efficiency reassembled 20.0 ± 6.1% almost same shown in solution (19.6 0.1%). shows that bi-fluorescent proteins silica particles retaining their properties.