Maedi-Visna virus : the development of serum and whole blood immunodiagnostic assays.

摘要: This thesis describes the development of serum and whole blood immunodiagnostic assays for Maedi-Visna virus (MVV). All previously described recombinant MVV ELISA utilised either core p25 or transmembrane (TM) proteins alone, combined, but as individual proteins. The TM genes were cloned individually into pGEX-2T expression vector. Both expressed a combined fusion protein in frame with glutathione S-transferase (GST). purified antigens (GST -TM GST -TM-p25) used to develop ELISA. Sera from 46 positive negative sheep tested using -TM-'p25 ELISAs commercial EIA kit. A two-graph receiver operating characteristic (TG-ROC) analysis program was interpret data. GST-TM-p25 more sensitive than assay which is based on antigen alone specific showed sensitivity specificity 100%. human AIDS lentivirus glycoprotein portion envelope viral has been identified most consistently recognised by antibodies. There suggestive evidence that same applies protein, E. coli, comparable -TM-p25 lacks specificity. However, due hydrophobic nature purification required lengthy protocols. despite fact only truncated version expressed. prompted investigating an alternative system could possibly circumvent above mentioned problems. yeast